Enzyme kinetics

NAME OF THE COURSE Enzyme kinetics

Code

KTG315

Year of study

3.

Course teacher

Assoc Prof Olivera Politeo

Credits (ECTS)

4.0

Associate teachers

ScD Ivana Carev
Asst Prof Franko Burčul

Type of instruction (number of hours)

P S V T

30

15

0

0

Status of the course

Elective

Percentage of application of e-learning

0 %

COURSE DESCRIPTION

Course objectives

Acquisition of basic knowledge and skills in the field of enzyme kinetics.

Course enrolment requirements and entry competences required for the course

Enrolled in or passed the course Enzyme kinetics exercise

Learning outcomes expected at the level of the course (4 to 10 learning outcomes)

- Understand the basic principles of chemical kinetics.
- Understand the importance and role of the enzymes in the life of the cell.
- Understand and explain Mechaelis-Menten kinetics model.
- Experimentally determined parameters of the enzyme-catalysed reactions.
- Understand and explain the importance and role of inhibition of enzyme-catalyzed reactions.
- Explain the mechanisms of regulation of enzyme activity.

Course content broken down in detail by weekly class schedule (syllabus)

LECTURES:
Basic principles of chemical kinetics: order of reaction. The reaction rate constants. The influence of temperature on rate constants. (3) Enzymes as biological catalysts: Basic properties of the enzymes. Nomenclature and classification of enzymes. The specificity of the enzymes. The active site of the enzymes. (4) Cofactors and coenzymes. (2) The kinetics of enzyme-catalyzed reactions: Activation energy. Enzyme-substrate reaction. Michaelis - Menten equation. Experimental determination of Km and Vmax. Graph of the Michaelis-Menten equation: the Lineweaver-Burkov double-reciprocal plot. Hanes plot. Eadie-Hofstee plot. (3) Inhibition of enzymes. Competitive inhibition. Uncompetitive reversible inhibition. Mixed inhibition. Irreversible inhibition. (3) Effect of pH and temperature on enzyme activity. (2) Control of enzyme activity: Cooperativity. Allosteric interactions. Hill equation. (2) Catalytic strategies. (4) Mehanisms of regulation: Inhibition feedback, covalent modifications, proteolytic cleavage (3) Kinetics of multienzyme systems. (2) Fast reactions (2)
SEMINARS:
Basic principles of chemical kinetics (2) Enzymes as biocatalysts. (1) Cofactors and coenzymes. (1) Michaelis-Menten equation. (4) Inhibition of enzymes. (4) Effect of pH and temperature on enzyme activity. (1) Allosteric interactions. (1) Mehanisms of regulation of enzyme activity. (1)

Format of instruction:

Student responsibilities

Class attendance, preparing seminar papers and taking the final exam.

Screening student work (name the proportion of ECTS credits for eachactivity so that the total number of ECTS credits is equal to the ECTS value of the course):

Class attendance

0.0

Research

0.0

Practical training

0.0

Experimental work

0.0

Report

0.0

 

 

Essay

0.0

Seminar essay

1.0

 

 

Tests

0.0

Oral exam

0.5

 

 

Written exam

2.5

Project

0.0

 

 

Grading and evaluating student work in class and at the final exam

Activity during attendance, presentation of seminar papers and final exam.

Required literature (available in the library and via other media)

Title

Number of copies in the library

Availability via other media

J.M. Berg, J.L. Tymoczko, L. Stryer: Biokemija, 5th Ed, Školska knjiga Zagreb, 2013.

0

R.K. Murray, D.A. Bender, K.M. Botham, P.J. Kennelly, V.W. Rodwell, P.A. Weil, Harperova ilustrirana biokemija, Medicinska naklada, Zagreb, 2011.

0

D. Voet. J. G. Voet, C. W. Pratt, Fundamentals of Biochemistry, John Wiley & Sons, Inc., NY, Chichester, Weinheim, Brisbane, Singapore, Toronto, 1999.

0

Cornish-Bowden: Fundamentals of Enzyme Kinetics, Butterworth, London 1979

0

Optional literature (at the time of submission of study programme proposal)

Christopher K. Mathews and K. E. Van Holde: Biochemistry, Second edition, The Benjamin / Cummings Publishing Company Inc 1996.;
Thomas M. Devlin: Textbook of Biochemistry, Third Edition, Wiley and Sons Inc., New York, Chichester, Brisbane, Toronto, Singapore, 1992.

Quality assurance methods that ensure the acquisition of exit competences

Monitoring of quality assurance will be performed at three levels: (1) University, (2) Faculty Level by Quality Control Committee, (3) Level of teachers.

Other (as the proposer wishes to add)