| NAME OF THE COURSE |
Enzyme kinetics |
|---|
Code |
|
Course teacher |
Prof Olivera Politeo |
Credits (ECTS) |
4.0 |
|
Associate teachers |
ScD Ivana Carev
Asst Prof Franko Burčul |
Type of instruction (number of hours) |
|
|
Status of the course |
Elective |
Percentage of application of e-learning |
0 % |
|
| COURSE DESCRIPTION |
Course objectives |
Acquisition of basic knowledge and skills in the field of enzyme kinetics. |
Course enrolment requirements and entry competences required for the course |
Enrolled in or passed the course Enzyme kinetics exercise |
Learning outcomes expected at the level of the course (4 to 10 learning outcomes) |
- Understand the basic principles of chemical kinetics.
- Understand the importance and role of the enzymes in the life of the cell.
- Understand and explain Mechaelis-Menten kinetics model.
- Experimentally determined parameters of the enzyme-catalysed reactions.
- Understand and explain the importance and role of inhibition of enzyme-catalyzed reactions.
- Explain the mechanisms of regulation of enzyme activity. |
Course content broken down in detail by weekly class schedule (syllabus) |
LECTURES:
Basic principles of chemical kinetics: order of reaction. The reaction rate constants. The influence of temperature on rate constants. (3) Enzymes as biological catalysts: Basic properties of the enzymes. Nomenclature and classification of enzymes. The specificity of the enzymes. The active site of the enzymes. (4) Cofactors and coenzymes. (2) The kinetics of enzyme-catalyzed reactions: Activation energy. Enzyme-substrate reaction. Michaelis - Menten equation. Experimental determination of Km and Vmax. Graph of the Michaelis-Menten equation: the Lineweaver-Burkov double-reciprocal plot. Hanes plot. Eadie-Hofstee plot. (3) Inhibition of enzymes. Competitive inhibition. Uncompetitive reversible inhibition. Mixed inhibition. Irreversible inhibition. (3) Effect of pH and temperature on enzyme activity. (2) Control of enzyme activity: Cooperativity. Allosteric interactions. Hill equation. (2) Catalytic strategies. (4) Mehanisms of regulation: Inhibition feedback, covalent modifications, proteolytic cleavage (3) Kinetics of multienzyme systems. (2) Fast reactions (2)
SEMINARS:
Basic principles of chemical kinetics (2) Enzymes as biocatalysts. (1) Cofactors and coenzymes. (1) Michaelis-Menten equation. (4) Inhibition of enzymes. (4) Effect of pH and temperature on enzyme activity. (1) Allosteric interactions. (1) Mehanisms of regulation of enzyme activity. (1) |
Format of instruction: |
|
Student responsibilities |
Class attendance, preparing seminar papers and taking the final exam. |
Screening student work (name the proportion of ECTS credits for eachactivity so that the total number of ECTS credits is equal to the ECTS value of the course): |
Class attendance |
0.0 |
Research |
0.0 |
Practical training |
0.0 |
Experimental work |
0.0 |
Report |
0.0 |
|
|
Essay |
0.0 |
Seminar essay |
1.0 |
|
|
Tests |
0.0 |
Oral exam |
0.5 |
|
|
Written exam |
2.5 |
Project |
0.0 |
|
|
|
Grading and evaluating student work in class and at the final exam |
Activity during attendance, presentation of seminar papers and final exam. |
Required literature (available in the library and via other media) |
Title |
Number of copies in the library |
Availability via other media |
J.M. Berg, J.L. Tymoczko, L. Stryer: Biokemija, 5th Ed, Školska knjiga Zagreb, 2013. |
0 |
|
R.K. Murray, D.A. Bender, K.M. Botham, P.J. Kennelly, V.W. Rodwell, P.A. Weil, Harperova ilustrirana biokemija, Medicinska naklada, Zagreb, 2011. |
0 |
|
D. Voet. J. G. Voet, C. W. Pratt, Fundamentals of Biochemistry, John Wiley & Sons, Inc., NY, Chichester, Weinheim, Brisbane, Singapore, Toronto, 1999. |
0 |
|
Cornish-Bowden: Fundamentals of Enzyme Kinetics, Butterworth, London 1979 |
0 |
|
|
Optional literature (at the time of submission of study programme proposal) |
Christopher K. Mathews and K. E. Van Holde: Biochemistry, Second edition, The Benjamin / Cummings Publishing Company Inc 1996.;
Thomas M. Devlin: Textbook of Biochemistry, Third Edition, Wiley and Sons Inc., New York, Chichester, Brisbane, Toronto, Singapore, 1992.
|
Quality assurance methods that ensure the acquisition of exit competences |
Monitoring of quality assurance will be performed at three levels: (1) University, (2) Faculty Level by Quality Control Committee, (3) Level of teachers. |
Other (as the proposer wishes to add) |
|
