Biochemistry

NAME OF THE COURSE Biochemistry

Code

KTC110

Year of study

1.

Course teacher

Assoc Prof Olivera Politeo

Credits (ECTS)

7.0

Associate teachers

Type of instruction (number of hours)

P S V T

30

15

30

0

Status of the course

Mandatory

Percentage of application of e-learning

0 %

COURSE DESCRIPTION

Course objectives

Acquisition a basic knowledge and skills in the field of biochemistry

Course enrolment requirements and entry competences required for the course

 

Learning outcomes expected at the level of the course (4 to 10 learning outcomes)

After passing the exam, student is expected to be able to:
- Understand the basic principles of protein structure and the impact of their structure on biological function.
- Understand the basic principles of enzyme kinetics and enzyme inhibition.
- Understand the importance of carbohydrate structures in the living system.
- Understand the importance of lipid structures in living system.
- Explain the structure and role of biological membranes.
- Understand the structure and biological function of nucleic acids.

Course content broken down in detail by weekly class schedule (syllabus)

LECTURES:
The history of biochemistry. The origin of life. (2) Water, bioelements, biomolecules and chemical bonds in living organisms. (2). Amino acids (1). Buffers & Buffer systems. (1). Proteins. Protein conformation. (2) Protein sequencing. (1) Protein function. Collagen & Elastin. Hemoglobin & Myoglobin. (2) Isolation and characterisation of proteins. (2) Enzymes. Enzyme kinetics. Enzyme inhibition. (2) Regulation of enzyme activity. (1) Coenzymes and Cofactors. (1) Carbohydrates. (2) Lipids. Lipoproteins. Steroids. (2). Biological membranes. Transport across membranes. (2) Nucleotides and nucleic acids. DNA replication. Transcription. Translation (3) Protein modifications and protein transport. (1) Bioenergetics. Energy-reach compounds: phosphate compounds and ATP. (3)
SEMINARS:
Buffers & Buffer systems. (3) Amino acids and peptides. (3) ) Enzymes. Enzyme kinetics. Enzyme inhibition. (3) Coenzymes (1) Carbohydrates (1) Lipids. (1) Biological membranes. (1) ) Nucleotides and nucleic acids (1) Bioenergetics. (1)
EXERCISES:
Izolacija i kvantitativno određivanje nukleinskih kiselina iz biljnih tkiva (3) The potentiometric titration of amino acids (3) Qualitative analysis of proteins (3) Quantitative determination of proteins by Bradford method. (3) Protein electrophoresis (3) The enzyme kinetics: determination vmax and Km. (3) Enzyme catalysis - effect of inhibitors on enzyme activity (3) The properties of carbohydrates and qualitative tests for carbohydrates (3) Properties of lipids, solubility, saponification (3) Lipid-isolation and detection of phospholipids from egg yolk (3) Isolation and quantification of nucleic acids from plant material (3)

Format of instruction:

Student responsibilities

 

Screening student work (name the proportion of ECTS credits for eachactivity so that the total number of ECTS credits is equal to the ECTS value of the course):

Class attendance

0.5

Research

0.0

Practical training

0.0

Experimental work

0.5

Report

0.0

 

 

Essay

0.0

Seminar essay

1.0

 

 

Tests

2.0

Oral exam

1.0

 

 

Written exam

1.0

Project

0.0

 

 

Grading and evaluating student work in class and at the final exam

Required literature (available in the library and via other media)

Title

Number of copies in the library

Availability via other media

J.M. Berg, J.L. Tymoczko, L. Stryer: Biokemija, 5th Ed, Školska knjiga Zagreb, 2013.

5

R.M.Murray i sur: Harperova ilustrurana biokemija, 28th Ed, Medicinska naklada, Zagreb 2011.

3

S K Sawhney, R ingh: Introductory Practical Biochemistry. Alpha Science International Ltd., Harrow, U.K. 2008.

1

Optional literature (at the time of submission of study programme proposal)

 

Quality assurance methods that ensure the acquisition of exit competences

Quality assurance will be performed at three levels:
(1) University Level;
(2) Faculty Level by Quality Control Committee;
(3) Lecturer’s Level.

Other (as the proposer wishes to add)