Enzyme kinetics

Code

Course teacher

Associate teachers

Status of the course

Course objectives

Acquisition of basic knowledge and skills in the field of enzyme kinetics.

Course enrolment requirements and entry competences required for the course

Enrolled in or passed the course Enzyme kinetics exercise

Learning outcomes expected at the level of the course (4 to 10 learning outcomes)

- Understand the basic principles of chemical kinetics.
- Understand the importance and role of the enzymes in the life of the cell.
- Understand and explain Mechaelis-Menten kinetics model.
- Experimentally determined parameters of the enzyme-catalysed reactions.
- Understand and explain the importance and role of inhibition of enzyme-catalyzed reactions.
- Explain the mechanisms of regulation of enzyme activity.

Course content broken down in detail by weekly class schedule (syllabus)

LECTURES:
Basic principles of chemical kinetics: order of reaction. The reaction rate constants. The influence of temperature on rate constants. (3) Enzymes as biological catalysts: Basic properties of the enzymes. Nomenclature and classification of enzymes. The specificity of the enzymes. The active site of the enzymes. (4) Cofactors and coenzymes. (2) The kinetics of enzyme-catalyzed reactions: Activation energy. Enzyme-substrate reaction. Michaelis - Menten equation. Experimental determination of Km and Vmax. Graph of the Michaelis-Menten equation: the Lineweaver-Burkov double-reciprocal plot. Hanes plot. Eadie-Hofstee plot. (3) Inhibition of enzymes. Competitive inhibition. Uncompetitive reversible inhibition. Mixed inhibition. Irreversible inhibition. (3) Effect of pH and temperature on enzyme activity. (2) Control of enzyme activity: Cooperativity. Allosteric interactions. Hill equation. (2) Catalytic strategies. (4) Mehanisms of regulation: Inhibition feedback, covalent modifications, proteolytic cleavage (3) Kinetics of multienzyme systems. (2) Fast reactions (2)
SEMINARS:
Basic principles of chemical kinetics (2) Enzymes as biocatalysts. (1) Cofactors and coenzymes. (1) Michaelis-Menten equation. (4) Inhibition of enzymes. (4) Effect of pH and temperature on enzyme activity. (1) Allosteric interactions. (1) Mehanisms of regulation of enzyme activity. (1)

Format of instruction:

Student responsibilities

Class attendance, preparing seminar papers and taking the final exam.