Methods of separation and speciation

NAME OF THE COURSE Methods of separation and speciation



Year of study


Course teacher

Prof Josipa Giljanović

Credits (ECTS)


Associate teachers

Type of instruction (number of hours)






Status of the course


Percentage of application of e-learning

0 %


Course objectives

Course enrolment requirements and entry competences required for the course

Completing the course: General Chemistry

Learning outcomes expected at the level of the course (4 to 10 learning outcomes)

1. Adoption of fundamental knowledge about the species and speciation analysis
2. Understand and apply the methods of sample preparation for analysis of species
3. Implement the instrumental methods in the analysis of complex species sample.
4. Implement the principles of validation of the analytical method in the analysis of species

Course content broken down in detail by weekly class schedule (syllabus)

1. Week: Analytical system. Sampling processing samples for analysis species. Seminar: Solving numerical examples treated theoretical material.
2. Week: Preparation of the sample for analysis species, validation, measurement uncertainty for sampling Seminar: Solving problems.
3. week: The importance and definition of speciation., Structural areas of speciation, isotopic composition, electronic and oxidation states. Seminar: Solving problems
4. Week: speciation of inorganic and organic compounds and complexes, organometallic compounds, speciation macromolecular compounds. Seminar: Solving problems
5. Week: Methodology specijacijske analysis. Seminar: Solving problems
6. week: Methods and techniques for speciation analysis and application. Seminar: Solving problems
7. Week 1.parcijalni test, solving test
8. week: The standard methods and reference materials, validation. Seminar: Solving problems
9. week: Application spectrometry as a detection system in the analysis of species. Seminar: Solving problems
10. week: Validation of spectroscopic methods and anliza species. Seminar: Solving problems
11. Week: Ion-selective electrode application in the analysis of species. Seminar: Solving problems
12. Week: Analysis of speciation, an example from the environment. Seminar: Solving problems 13 Week. Analysis of speciation, examples of analyzes of food. Seminar: Solving problems
14. Week: flow-injection method, an example of an analysis of species. An automated analytical systems. Seminar: Solving problems
15th week: Examination (II. Partial test theoretical and seminar materials). Solving test
List of exercises:
1. Gravimetric determination of iron species
2. Spectrophotometric determination of iron species
3. Gravimetric determination of chromium species
4. Spectrophotometric determination of chromium species
5. Determination of EDTA species

Format of instruction:

Student responsibilities

Lectures attendance - at least 80% and completing exercises

Screening student work (name the proportion of ECTS credits for eachactivity so that the total number of ECTS credits is equal to the ECTS value of the course):

Class attendance




Practical training


Experimental work








Seminar essay






Oral exam




Written exam






Grading and evaluating student work in class and at the final exam

The entire test can be applied over two partial tests during the semester. Passing threshold is 60%. Each test in assessing participates with 50%. Lectures presence of 80 to 100% is 10% marks. The examination periods there is a written and oral exam. Passing threshold is 60%. Passing one partial test of any part (previous activity) is valid throughout current academic year. Written exam has a share of 50% and oral examination also 50%. Students who have not passed the partial tests will have oral examination in the regular examination period. Passing threshold is 60% and the examination form to participate in the evaluation by 50%.
Rating: 60% -69% - satisfactory, 70% -79% - good, 80% -89% very good, 90% -100% - excellent.

Required literature (available in the library and via other media)


Number of copies in the library

Availability via other media

D. A.Skoog, D. M. West, F. J. Holler, Fundamentals of Analytical Chemistry, Seventh Edition, Saunders College Publishing, New York, London, 1996. {šesto izdanje (englesko) 1992, prvo izdanje (hrvatsko), Školska knjiga, Zagreb, 1999.}


A. Skoog, D. M. West i F. J. Holler, S. R. Crouch, Fundamentals of Analytical Chemistry, 9th edition, Brooks&Cole, SAD, 2014.; 3.D.C.Harris, Quantitative Chemical Analysis, Eighth Edition, W.H.Freeman and Company, New York, 2010.


na web-stranici Zavoda za analitičku kemiju

R. Cornelis et al. Handbook of Elemental Speciation: Techniques and Methodology, Book News, Portland, 2004.


Optional literature (at the time of submission of study programme proposal)


Quality assurance methods that ensure the acquisition of exit competences

- registration of student’s presence in class
- annual analysis of students success in this course
- student’s survey in order to evaluate the professor
- professor’s self-evaluation

Other (as the proposer wishes to add)