Chemical sensors and biosensors

NAME OF THE COURSE Chemical sensors and biosensors

Code

KTH218

Year of study

2.

Course teacher

Asst Prof Marijo Buzuk

Credits (ECTS)

3.0

Associate teachers

Type of instruction (number of hours)

P S V T

30

0

15

0

Status of the course

Elective

Percentage of application of e-learning

0 %

COURSE DESCRIPTION

Course objectives

Students will get insight into actual achievements in chemical and biochemical sensing systems. Basic principles and kind of chemical and biochemical sensor systems will be presented together with problems that can arise during development process of such sensors systems (sensors material or biomaterial, recognition mechanism, choosing appropriate transducer element, configuration and practical development of sensors. Furthermore, practical applicability of chemical sensors and biosensors in medical, industrial and environmental analysis will be given.

Course enrolment requirements and entry competences required for the course

 

Learning outcomes expected at the level of the course (4 to 10 learning outcomes)

1. To recognize and characterize sensors systems.
2. To propose and to development novel sensors systems, together with improvement of current ones.
3. To use a knowledge in selection of appropriate sensors systems for various analytes in different area of application.
4. To get specific insight into recognition principles, amplification and transduction of chemical signal into various outcome signals.

Course content broken down in detail by weekly class schedule (syllabus)

1. General aspects and introduction in chemical sensors and biosensors-definition, terminology.
Classification of sensors (electrochemical, optical, piezoelectrical, termal, aqoustic). Phenomenon (recognition mechanism) that can be applied in sensors systems. Novel trends and scopes in sensors development (nanotechnology, nanostructure, ”smart” sensors, in vivio sensors)
2. Basic construction concepts of sensors and biosensors. Example of commercial sensors and biosensors (ion-selective electrodes, glucometer, Elisa assay). Chemical and biochemical recognition mechanism. Recognition elements: ionophores, enzymes, crystals. Criterion for recognition elements selection. Biochemical selectivity and stability of recognition elements-problems.
3. Matrix material of sensors. Role and characteristics of matrixes. Recognition element immobilization methods. Incorporation of recognition elements into various matrixes (gels, polymers: conducting or non-conducting, carbon materials: paste, screen-printing, carbon nanotubes). Covalent binding of enzymes, cross-linking, physical adsorption.
4. Transducer for electrochemical, optical, piezoelectrical sensors. Selection of transducing element. Electrochemical sensors: potentiometric, amperometric. Modiffied electrodes. Preparation of ion-selective electrodes-techniques. Conducting surface, modified layer, signal transducing. Microelectrodes, FET sensors.
5. Optical sensors. Absorption, UV absorption, reflectometry, luminescence. Optical fibers sensors. Intrinsic and extrinsic sensors. Application of optical sensors in sensing of pH, pressure, temperature, humidity. Determination of biomolecules with optical sensors-luciferase in ATP detection and bacterial redox systems with luciferase in NADPH detection.
6. Piezoelectrical sensors. General aspects. Acoustic sensors. Incorporation of recognition elements.
7. Biosensors. Electrochemical, optical, immunosensors, optical sensors. First, second and third generation of biosensors.
8. Examples of the three generation of biosensonsors-amperometric determination of glucose. Redox interference. Oxygen dependence of the signal. Peroxide role. in vitro and in vivo continuously monitoring of glucose. Modern scopes in development of glucometers. Commercialization of sensors.
9. Immunosensors. Antibody-antigen bonding as mechanism of recognition. Electrochemical, optical, immunosensors, optical immunosensors. Examples of immunosensors for hemoglobin, erythrocytes, t-lymphocytes, viruses and bacterial. ELISA test.
10. DNA detection. DNA electrochemical sensors. Electrochemistry of DNA and detection principles. Immobilization of DNA sequences onto different materials. Electrodes processes-hybridization. Enzyme labeled DNA sequences.
11. Bionseors for pesticide detection. Enzymes used for pesticide detection. Basic concepts. Inhibition mechanism. Catalytically based biosensors. Immunosensors for pesticides determination. Enzymes reactivation. Microorganism, issue, cells as recognition elements.
12. Nanomaterial in optical and electrochemical biosensors. Carbon nanotubes (CNT) in biosensors preparation. CNT composite materials.
13. Introduction in direct transfer of electrons by proteins. Protein immobilization techniques. Examples: cytochrome c, myoglobine, hemoglobine. Role of CNT. Direct electron transfer from enzyme onto electrode. Examples: glucose oxidase, catalase, HRP.
14. Evaluation of sensors: selectivity, sensitivity, reproducibility, repeatability. Other types of sensors: impedance sensors, sensors for gases.
15. Highly sophisticated sensors systems: microelectro-machanical systems (MEMS and Bio(MEMS)), Lab-on-a-chip, nanosensors, biochips.
Lab courses:
1. Preparation of potentiometric sensors by incorporation of ionophores in polymer matrix. Components ratio influences onto sensor characteristics. Determination of heavy metals in water. Selectivity of prepared sensor.
2. Amperometric determination of amino acids onto crystal and bismuth modified electrodes. Electrode preparation, characterization, influences of parameters on signal quality.
3. Preparation of the biosensors for determination of glucose. Incorporation of glucoso oxidase into carbon paste. MnO2 as transducing element. Interferences. Role of Nafion® in improvment of the signal. Determination of glucose in real samples

Format of instruction:

Student responsibilities

The 80% presence at lectures and completed all laboratory exercises.

Screening student work (name the proportion of ECTS credits for eachactivity so that the total number of ECTS credits is equal to the ECTS value of the course):

Class attendance

0.5

Research

0.0

Practical training

0.0

Experimental work

1.0

Report

0.0

 

 

Essay

0.0

Seminar essay

0.0

 

 

Tests

0.0

Oral exam

2.0

 

 

Written exam

0.5

Project

0.0

 

 

Grading and evaluating student work in class and at the final exam

Prior to joining the laboratory exercises, students’ knowledge of the material concerned exercises will be verified by tests. All exercises must be completed.
The exam consists of a written examination. The written part of the exam lasts two hours. The written part of the exam is evaluated as follows:
Exactly solved more than 55 % - sufficient
Exactly solved more than 70 % - good
Exactly solved more than 80 % - very good
Exactly solved more than 90 % - excellent
A complete examination or part thereof may be installed through two partial tests during the semester. The tests cover material presented in lectures and lab courses. Written tests are evaluated in the following manner:
Exactly solved more than 55 % - released a written exam
Exactly solved by 60 % - freed written and oral - sufficient
Exactly solved by 70 % - freed written and oral - good
Exactly solved by 80 % - freed written and oral - very good
Exactly solved by 90 % - freed written and oral - excellent
It is necessary to pass all tests in order to pass the exam. Students who did not meet any of the tests must take written and oral exam of that part.
Also, essays make a basis for discussions and are rooted in the central parts of the syllabus for the course in question. Essays can be written on the basis of the course literature and lectures and will be presented to all course participants.

Required literature (available in the library and via other media)

Title

Number of copies in the library

Availability via other media

Florinel-Gabriel Bănică, Chemical Sensors and Biosensors: Fundamentals and Applications, John Wiley & Sons, Ltd, 2012.

0

E. Palecek, F. Scheller, J. Wang Electrochemistry of Nucleic Acids and Proteins, Elsevier, 2005.

0

Xueji Zhang, Huangxian Ju, Joseph Wang, Electrochemical Sensors, Biosensors and Their Biomedical Applications, Elsevier, 2008.

0

E. Turkušić, Uvod u hemijske senzore i biosenzore, PMF Sarajevo, 2012

0

Optional literature (at the time of submission of study programme proposal)

 

Quality assurance methods that ensure the acquisition of exit competences

- Information from interviews, observations, and consultation with students during lectures
- Student survey

Other (as the proposer wishes to add)