Exercises in Biochemistry 1

NAME OF THE COURSE

Code

KTG305

Year of study

Course teacher

Assoc Prof Mila Radan

Credits (ECTS)

4.0

Associate teachers

Type of instruction (number of hours)

P	S	V	T
0	0	60	0

Status of the course

Mandatory

Percentage of application of e-learning

0 %

COURSE DESCRIPTION

Course objectives

This laboratory course will introduce student to techniques fundamental to biochemical investigations.

Course enrolment requirements and entry competences required for the course

Enrolled in or passed the course Biochemistry I

Learning outcomes expected at the level of the course (4 to 10 learning outcomes)

- understand and use many of the techniques and tools of biochemistry
- comprehend fundamental principles of biochemical research
- communicate research results effectively in written format

Course content broken down in detail by weekly class schedule (syllabus)

Potentiometric determination of amino acids (4) Qualitative analysis of proteins: colored and precipitation reactions to proteins (4) Lipid-isolation and detection of phospholipids from egg yolk (4) Properties of carbohydrates and carbohydrate tests (4) Enzymes: properties of digestive enzymes - ptyalin (4) Determination of protein concentration by Bradford assay (4) Determination of carbohydrate structures using periodic acid (4) Properties of lipids, solubility, saponification (4) Gel filtration of hemoglobin (4) Enzyme catalysis - kinetics of the enzymatic reactions (4) Enzyme catalysis - effect of inhibitors on enzyme activity (4) enzyme catalysis - effect of temperature and pH on enzyme activity (4) Isolation and quantification of nucleic acids (4) Protein electrophoresis (4) Biological oxidation: oxidation test with substance (4)

Format of instruction:

Student responsibilities

Screening student work (name the proportion of ECTS credits for eachactivity so that the total number of ECTS credits is equal to the ECTS value of the course):

Class attendance	0.0	Research	0.0	Practical training	0.0
Experimental work	3.0	Report	1.0
Essay	0.0	Seminar essay	0.0
Tests	0.0	Oral exam	0.0
Written exam	0.0	Project	0.0

Grading and evaluating student work in class and at the final exam

Required literature (available in the library and via other media)

Title	Number of copies in the library	Availability via other media
Interna skripta "Biokemijski praktikum", O. Politeo	0

Optional literature (at the time of submission of study programme proposal)

Quality assurance methods that ensure the acquisition of exit competences

Quality of the teaching and learning, monitored at the level of the
(1) teachers, accepting suggestions of students and colleagues, and
(2) faculty, conducting surveys of students on teaching quality.

Other (as the proposer wishes to add)