Biochemistry 1

NAME OF THE COURSE Biochemistry 1

Code

KTG304

Year of study

3.

Course teacher

Asst Prof Mila Radan

Credits (ECTS)

5.0

Associate teachers

Type of instruction (number of hours)

P S V T

45

15

0

0

Status of the course

Mandatory

Percentage of application of e-learning

0 %

COURSE DESCRIPTION

Course objectives

Acquisition of basic knowledge and skills in the field of biochemistry.

Course enrolment requirements and entry competences required for the course

Enrolled in or passed the course Biochemistry I laboratory

Learning outcomes expected at the level of the course (4 to 10 learning outcomes)

(1) Upon completion of this course, students should be able to recognize how fundamental chemical principles and reactions are utilized in biochemical processes. (2) Be able to identify, formulate and solve complex biochemical problems. (3) Calculate the speed of biochemical reactions catalyzed by enzymes, determine the type of inhibition (4) To predict the potential impact of compounds on the pace of enzymatic reactions (5) Have the necessary knowledge and strategies for the separation, identification and quantification of compounds and elements from complex mixtures. (6) Connect the structure of DNA molecules with the transfer of genetic information and the principles of inheritance (7) Be able to design and conduct experiments. (8) Be able to communicate the findings and conclusions. (9) Read and understand original research.

Course content broken down in detail by weekly class schedule (syllabus)

The contents of the course, the historical development of biochemistry, biomolecules, biological structures (3) Water, hydrogen bonds, the role of water, buffer systems in the body, amino acids (3) Amino acids, peptides, proteins, peptide bond (4) The techniques of protein purification; electrophoresis, chromatographic methods, ultracentrifugation (4) Characterization of protein: Edman degradation, immunological, mass spectrometry, MALDI-TOF, X-ray crystallography, NMR spectroscopy (3) Structure of nucleotides, DNA, RNA, and the flow of genetic information, DNA replication, transcription and translation, gene expression (3) Hemoglobin-myoglobin, structure and function, allosteric proteins (3) Enzymes as catalysts in biological systems, the model Menten kinetics Michaelis- (3) Types of enzyme inhibition: competitively, nekompeticijski and akompeticijski inhibitors (3) Catalytic strategies, catalytic core principles, covalent catalysis, acid base catalysis, catalysis approaching, metal ion catalysis (3) Regulatory strategies, allosteric control, isozymes, reversible covalent modification, activation of proteolysis-zymogens (3) Carbohydrates: monosaccharides, complex carbohydrates, glycoproteins, proteoglycans, lectins (3) lipids and the cell membrane, transport of molecules through the membrane, types of movement across the cell membrane (4) Signal transduction cascades, primary and secondary signal carriers, phosphorylation cascade, disturbances in the signal conducting paths (3)

Format of instruction:

Student responsibilities

 

Screening student work (name the proportion of ECTS credits for eachactivity so that the total number of ECTS credits is equal to the ECTS value of the course):

Class attendance

1.0

Research

0.0

Practical training

0.0

Experimental work

0.0

Report

0.0

 

 

Essay

0.0

Seminar essay

1.0

 

 

Tests

1.0

Oral exam

1.0

 

 

Written exam

1.0

Project

0.0

 

 

Grading and evaluating student work in class and at the final exam

Partial exams. Oral examination.

Required literature (available in the library and via other media)

Title

Number of copies in the library

Availability via other media

Biokemija , J.M. Berg, J.L. Tymoczko and L. Stryer, Prijevod VI izdanja, Školska knjiga Zagreb, 2013.

1

Optional literature (at the time of submission of study programme proposal)

 

Quality assurance methods that ensure the acquisition of exit competences

Quality of the teaching and learning, monitored at the level of the
(1) teachers, accepting suggestions of students and colleagues, and
(2) faculty, conducting surveys of students on teaching quality.

Other (as the proposer wishes to add)