Instrumental Methods of Analysis

NAME OF THE COURSE Instrumental Methods of Analysis

Code

KTB103

Year of study

1.

Course teacher

Asst Prof Lea Kukoč Modun

Credits (ECTS)

6.5

Associate teachers

ScD Maja Biočić
Asst Prof Franko Burčul

Type of instruction (number of hours)

P S V T

30

15

30

0

Status of the course

Mandatory

Percentage of application of e-learning

0 %

COURSE DESCRIPTION

Course objectives

The aim of this course is to introduce students to the theoretical principles, practical work and the use of instrumental techniques and procedures relating to the process analysis. The choice of method will depend on the knowledge of the basic principles of individual method or group of methods and the understanding of their advantages and limitations. After completion of a process of learning the learner is able for independent work in instrumental analytical laboratory.

Course enrolment requirements and entry competences required for the course

 

Learning outcomes expected at the level of the course (4 to 10 learning outcomes)

1. Adopt theoretical knowledge related to methods of instrumental analysis (spectrometry, electroanalytical, thermal methods, instrumental separation methods) and the principles of instruments.
2. Correctly interpret the adopted theoretical knowledge relating to methods of analysis instrument and principles of instruments.
3. Explain the connection between basic knowledge of analytical chemistry with application in instrument analysis.
4. Select analytical technique due to the characteristics of the analyte and the specificity of the sample.
5. Integrate acquired knowledge and apply them in problem-solving and decision-making in analytical practice and in process analysis.
6. Adopt theoretical knowledge related to methods of instrumental analysis ( spectrometry , electroanalytical , thermal methods , instrumental methods for separation ) and principles of instruments and apply knowledge in the experimental work.
7. Select analytical technique due to the characteristics of the analyte and the specificity of the sample.
8. Plan and install an experiment using instrumental techniques.
9. Apply basic statistical analysis of numerical data and graphed the results.
10. Independently take Lab Notes and prepare a report after completion of the analysis.

Course content broken down in detail by weekly class schedule (syllabus)

1st week
Lectures: Fundamentals of instrumental techniques and their application in continuous and process analysis.
Seminars: Introduction, memento. SI system of units.
2nd week
Lectures: Planning and optimizing the experiment. Optimizing analytical control of technology process.
Seminars: Kinetic method analysis.
3rd week
Lectures: Gass chromatography. High performance liquid chromatography. Gass chromatography coloumns and detectors.
Seminars: Chromatography (numerical examples).
4th week
Lectures: Continuous segmentation flow analysis. Flow injection analysis.
Seminars: Flow injection analysis, construction of manifold.
5th week
Lectures: Thermal analysis Termogravimetric methods. Differential thermal analysis.
Seminars: Thermal analysis (numerical examples).
6th week
Lectures: Fundamentals of spectrophotometry. Atomic absorption spectrometry. Flame emission spectrometry. Atomic fluorescence. Atomic emission. Atomic absorption.
Seminars: Atomic absorption spectroscopy.
7th week
Lectures: Ultraviolet / Visible absorption spectrometry.
Seminars: Spectrometry (numerical examples).
8th week
Lectures: Infrared absorption spectrometry. Raman spectrometry.
Seminars: Spectrometry (numerical examples).
11th week
9th week
Lectures: Mass spectrometry. Nuclear Magnetic Resonance Spectrometry, Fotoelectron spectrometry. Auger electron spectrometry. Photoelectron spectroscopy. Analysis of surface with electron beams.
Seminars: Mass spectrometry, modern ionisation methods.
10th week
Lectures: Microanalysis with electronic sampling. X-ray diffraction analysis. Scanning electron microskop.
Seminars: Potentiometry (numerical examples).
11th week
Lectures: Electroanalytical methods. Potentiometry. Indicator electrodes. Potentiometric setup.
Seminars: Potentiometry (numerical examples).
12th week
Lectures: Coulometry.
Seminars: Electrogravimetry (numerical examples).
13th week
Lectures: Coulometry
Seminars: Coulometry (numerical examples).
14th week
Lectures: Voltammetry.
Seminar: Voltammetry (numerical examples).
15th week
Lectures: Amperometry.
Seminars: Amperometry (numerical examples).
Experimantal part:
1. Kinetic methods of analysis, determoination of tiolic compound using kinetic manifold with spectrophotometric detector.
2. Flow injection analysis, determination of ascorbic acid by flow injection analysis and spectrophotometric detector.
3. UV/Vis spectrophotometry, spectrophotometric measurement of an equilibrium constant.
4. Atomic absorption spectroscopy, determination of metals in real samples.
5. Ions selective electrode, potentiometry, measurement of an equilibrium constant.
6. Electrogravimetric determination, determination or separation of metals.

Format of instruction:

Student responsibilities

 

Screening student work (name the proportion of ECTS credits for eachactivity so that the total number of ECTS credits is equal to the ECTS value of the course):

Class attendance

0.0

Research

0.0

Practical training

0.0

Experimental work

0.3

Report

0.0

Test numeričkih zadataka

2.0

Essay

0.0

Seminar essay

0.0

Teorijski dio testa

3.3

Tests

0.0

Oral exam

1.0

 

 

Written exam

0.0

Project

0.0

 

 

Grading and evaluating student work in class and at the final exam

Scoring at the exam consists of three basic parts: scoring the experimental part (minimum score 2 , maximum score 4), test of numerical example (minimum score: 18; maximum score: 30) and test of theoretical part (minimum score: 39; maximum score: 65).
Students who had attended lectures and seminar in 70 % can take the exam through partial tests: 2 tests of numerical examples (minimum score: 9; maximum score: 15) and 2 tests of theoretical part (minimum score: 19,5; maximum score: 32,5).
The rating is formed in accordance with the score ranges: sufficient ( 60 - 70 points) , good ( 71-80 points) , very good ( 81-90 points) , excellent ( ≥91points ).

Required literature (available in the library and via other media)

Title

Number of copies in the library

Availability via other media

D.A. Skoog, D.M. West, F.J. Holler, Osnove analitičke kemije, šesto izdanje (englesko), prvo izdanje (hrvatsko), Školska knjiga, Zagreb, 1999.

18

Nj. Radić i L. Kukoč Modun, Uvod u analitičku kemiju, Školska knjiga, Zagreb, 2016.

4

M. Kaštelan-Macan, Kemijska analiza u sustavu kvalitete, Školska knjiga, Zagreb 2003.

2

I. Piljac, Elektroanalitičke metode, RMC, 1995.

3

I. Piljac, Senzori fizikalnih veličina i analitičke metode, Zagreb, 2010.

3

Analitika okoliša (ur. M. Kaštelan Macan, M. Petrović), HINUS i FKIT, Zagreb 2013.

3

I. S. Krull, Analytical Chemistry, Intech, Rijeka, 2012.

0

dostupno na webu: DOI: 10.5772/3086

M. Kaštelan-Macan, M. Medić-Šarić, S. Turina, Plošna kromatografija, Farmaceutsko-biokemijski fakultet, Zagreb. 2006.

20

T. Bolanča, Š. Ukić, Ionska kromatografija, Fakultet kemijskog inženjerstva i tehnologije, Zagreb, 2015.

0

dostupno na webu: https://www.fkit.unizg.hr/images/50012393/Bolanca-Ukic_Ionska_kromatografija.pdf

L. Kukoč, Molekulska spektroskopija, Interna recenirana skripta, 2003.

30

dostupno u digitalnom obliku

L. Kukoč, Spektrometrijske metode elementne analize, Interna recenirana skripta, 2005.

30

dostupno u digitalnom obliku

Josipa Komljenović, Ion selektivna sulfidna elektroda, Interna recenzirana skripta

30

Josipa Komljenović, Ion selektivna sulfidna elektroda, Interna recenzirana skripta

Optional literature (at the time of submission of study programme proposal)

R. Kellner, J. M. Mermet, M. Otto, M. Valcarcel and H. M. Widmer (Urednici), Analytical Chemistry (A Modern Approach to Analytical Science, Second Edition) Wiley-VCHVerlag Gmbh & Co. KGaA, Weinheim, 2004.
D. A. Skoog, D. M. West, F. J. Holler and S. R. Crouch, Fundamentals of Analytical Chemistry, Eighth Edition, Thompson Brooks/Cole, Belmont, USA, 2004.
G. D.Christian, Analytical Chemistry, Sixth Edition, John Willey & Sons, INC, 2004.
D. Harvey, Modern Analytical Chemistry, McGraw-Hill Higher Education, New York, London, 2000.
F. W. Fifield & D. Kealey, Principles and Practice of Analytical Chemistry, Blackwell Science Ltd, Ma

Quality assurance methods that ensure the acquisition of exit competences

Quality assurance will be performed at three levels:
(1) University Level;
(2) Faculty Level by Quality Control Committee;
(3) Lecturer’s Level.

Other (as the proposer wishes to add)