Learning outcomes expected at the level of the course (4 to 10 learning outcomes) |
- definition of term biotechnology
- differentiate the primary and secondary cell metabolism and its application in biotechnology
- explain of microbe cell growth diagram
- explain the advantages of isolated enzymes in biotechnology
- describe of basic bioreactor design
- describe the basic methods of intracellular products isolation
- outline some examples of biotechnological processes. |
Course content broken down in detail by weekly class schedule (syllabus) |
1st week: Description and overview of the course. Definitions of biotechnology, interdisciplinary of the field, history development, application areas.
2nd week: Perception of biotechnology in society. Metabolism and control of metabolic processes; primary and secondary metabolism, substrates.
3rd week: Anaerobic and aerobic metabolism. Microorganisms in biotechnology (bacterial, fungi (yeasts and moulds). Microbial growth kinetics. Determination of specific growth rate and Monod constant.
4th week: Enzyme technology (enzyme characteristics as the biocatalysts, advantages and disadvantages compared to whole cells). Enzyme kinetics.
5th week: Enzyme sources. Selection, production and immobilization of enzymes.
6th week: Biocatalysts in non-conventional processes. Bioreactors, photo-bioreactors, design.
7th week: Oxygen transfer and oxygen concentration determination. Heat transfer in bioreactor. An overview of the previous lecture for the test.
First test.
8th week: Extracellular, periplasmic and extracellular products of metabolism. Down-stream processing: separation solid-liquid, isolation of intracellular products.
9th week: Concentration and purification of products from bioreactor. Purification process control.
10th week: Process economy: cost estimation and an example of process design. An overview of biotechnological processes – basic scheme of biotechnological process.
11th week: Alcohol fermentation and its application in industry.
12th week: Lactic acid fermentation and its application in industry. Anaerobic biomass fermentation.
13th week: Biotechnology in pharmacy. Bacterial polymers.
14th week: Biotechnology in environmental protection and waste water treatment. Biosensors.
15th week: An overview of the previous lecture for the test. Second test. |
