Physical biochemistry

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Course objectives

The goal of course Physical biochemistry is to connect and comprehensive understanding of the knowledge that students have gained learning courses of Physical Chemistry and Biochemistry.

Course enrolment requirements and entry competences required for the course

Learning outcomes expected at the level of the course (4 to 10 learning outcomes)

Understanding (1) the application of thermodynamic principles in biochemistry, (2) the concept of chemical equilibrium, (3) chemical kinetics, (4) electrochemical processes, (5) bioenergetics and membrane processes and (6) the modern methods in biochemistry.

Course content broken down in detail by weekly class schedule (syllabus)

Introduction, Concepts of Thermodynamics in Biochemistry (1 hour). Acids, bases and buffers in biochemistry (titration curves and electric charge, titration curves and protein isoelectric point) (1 hour). Thermodynamic concepts in biochemistry (open systems and the environment, work, energy and heat, the tool states, entropy and Gibbs free energy, thermodynamics and metabolism (2 hours). Bioenergetics and mebranski transfers (1 hour). Biochemical reactions and balance (1 hour) . Electrochemistry and biochemical processes (1 hour). interaction of protein - ligand (dissociation constant of singlet binding sites, dissociation constant of the inhibitor, the number of binding sites, thermodynamics of protein-ligand interactions (2 hours). Chemical kinetics of biochemical reactions, enzyme kinetics (2 hours) . Modern methods in biochemistry: Spectroscopic methods, methods of isolation, purification and identification of proteins, DNA molecules research methods, methods based on radioactivity (4 hours).

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Student responsibilities