Exercises in Biochemistry 1

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Status of the course

Course objectives

This laboratory course will introduce student to techniques fundamental to biochemical investigations.

Course enrolment requirements and entry competences required for the course

Enrolled in or passed the course Biochemistry I

Learning outcomes expected at the level of the course (4 to 10 learning outcomes)

- understand and use many of the techniques and tools of biochemistry
- comprehend fundamental principles of biochemical research
- communicate research results effectively in written format

Course content broken down in detail by weekly class schedule (syllabus)

Potentiometric determination of amino acids (4) Qualitative analysis of proteins: colored and precipitation reactions to proteins (4) Lipid-isolation and detection of phospholipids from egg yolk (4) Properties of carbohydrates and carbohydrate tests (4) Enzymes: properties of digestive enzymes - ptyalin (4) Determination of protein concentration by Bradford assay (4) Determination of carbohydrate structures using periodic acid (4) Properties of lipids, solubility, saponification (4) Gel filtration of hemoglobin (4) Enzyme catalysis - kinetics of the enzymatic reactions (4) Enzyme catalysis - effect of inhibitors on enzyme activity (4) enzyme catalysis - effect of temperature and pH on enzyme activity (4) Isolation and quantification of nucleic acids (4) Protein electrophoresis (4) Biological oxidation: oxidation test with substance (4)

Format of instruction:

Student responsibilities