Biochemistry 1

Code

Course teacher

Associate teachers

Status of the course

Course objectives

Acquisition of basic knowledge and skills in the field of biochemistry.

Course enrolment requirements and entry competences required for the course

Enrolled in or passed the course Biochemistry I laboratory

Learning outcomes expected at the level of the course (4 to 10 learning outcomes)

(1) Upon completion of this course, students should be able to recognize how fundamental chemical principles and reactions are utilized in biochemical processes. (2) Be able to identify, formulate and solve complex biochemical problems. (3) Calculate the speed of biochemical reactions catalyzed by enzymes, determine the type of inhibition (4) To predict the potential impact of compounds on the pace of enzymatic reactions (5) Have the necessary knowledge and strategies for the separation, identification and quantification of compounds and elements from complex mixtures. (6) Connect the structure of DNA molecules with the transfer of genetic information and the principles of inheritance (7) Be able to design and conduct experiments. (8) Be able to communicate the findings and conclusions. (9) Read and understand original research.

Course content broken down in detail by weekly class schedule (syllabus)

The contents of the course, the historical development of biochemistry, biomolecules, biological structures (3) Water, hydrogen bonds, the role of water, buffer systems in the body, amino acids (3) Amino acids, peptides, proteins, peptide bond (4) The techniques of protein purification; electrophoresis, chromatographic methods, ultracentrifugation (4) Characterization of protein: Edman degradation, immunological, mass spectrometry, MALDI-TOF, X-ray crystallography, NMR spectroscopy (3) Structure of nucleotides, DNA, RNA, and the flow of genetic information, DNA replication, transcription and translation, gene expression (3) Hemoglobin-myoglobin, structure and function, allosteric proteins (3) Enzymes as catalysts in biological systems, the model Menten kinetics Michaelis- (3) Types of enzyme inhibition: competitively, nekompeticijski and akompeticijski inhibitors (3) Catalytic strategies, catalytic core principles, covalent catalysis, acid base catalysis, catalysis approaching, metal ion catalysis (3) Regulatory strategies, allosteric control, isozymes, reversible covalent modification, activation of proteolysis-zymogens (3) Carbohydrates: monosaccharides, complex carbohydrates, glycoproteins, proteoglycans, lectins (3) lipids and the cell membrane, transport of molecules through the membrane, types of movement across the cell membrane (4) Signal transduction cascades, primary and secondary signal carriers, phosphorylation cascade, disturbances in the signal conducting paths (3)

Format of instruction:

Student responsibilities