Instrumental Methods of Analysis

Code

Course teacher

Associate teachers

Status of the course

Course objectives

The aim of this course is to introduce students to the theoretical principles, practical work and the use of instrumental techniques and procedures relating to the process analysis. The choice of method will depend on the knowledge of the basic principles of individual method or group of methods and the understanding of their advantages and limitations. After completion of a process of learning the learner is able for independent work in instrumental analytical laboratory.

Course enrolment requirements and entry competences required for the course

Learning outcomes expected at the level of the course (4 to 10 learning outcomes)

1. Adopt theoretical knowledge related to methods of instrumental analysis (spectrometry, electroanalytical, thermal methods, instrumental separation methods) and the principles of instruments.
2. Correctly interpret the adopted theoretical knowledge relating to methods of analysis instrument and principles of instruments.
3. Explain the connection between basic knowledge of analytical chemistry with application in instrument analysis.
4. Select analytical technique due to the characteristics of the analyte and the specificity of the sample.
5. Integrate acquired knowledge and apply them in problem-solving and decision-making in analytical practice and in process analysis.
6. Adopt theoretical knowledge related to methods of instrumental analysis ( spectrometry , electroanalytical , thermal methods , instrumental methods for separation ) and principles of instruments and apply knowledge in the experimental work.
7. Select analytical technique due to the characteristics of the analyte and the specificity of the sample.
8. Plan and install an experiment using instrumental techniques.
9. Apply basic statistical analysis of numerical data and graphed the results.
10. Independently take Lab Notes and prepare a report after completion of the analysis.

Course content broken down in detail by weekly class schedule (syllabus)

1st week
Lectures: Fundamentals of instrumental techniques and their application in continuous and process analysis.
Seminars: Introduction, memento. SI system of units.
2nd week
Lectures: Planning and optimizing the experiment. Optimizing analytical control of technology process.
Seminars: Kinetic method analysis.
3rd week
Lectures: Gass chromatography. High performance liquid chromatography. Gass chromatography coloumns and detectors.
Seminars: Chromatography (numerical examples).
4th week
Lectures: Continuous segmentation flow analysis. Flow injection analysis.
Seminars: Flow injection analysis, construction of manifold.
5th week
Lectures: Thermal analysis Termogravimetric methods. Differential thermal analysis.
Seminars: Thermal analysis (numerical examples).
6th week
Lectures: Fundamentals of spectrophotometry. Atomic absorption spectrometry. Flame emission spectrometry. Atomic fluorescence. Atomic emission. Atomic absorption.
Seminars: Atomic absorption spectroscopy.
7th week
Lectures: Ultraviolet / Visible absorption spectrometry.
Seminars: Spectrometry (numerical examples).
8th week
Lectures: Infrared absorption spectrometry. Raman spectrometry.
Seminars: Spectrometry (numerical examples).
11th week
9th week
Lectures: Mass spectrometry. Nuclear Magnetic Resonance Spectrometry, Fotoelectron spectrometry. Auger electron spectrometry. Photoelectron spectroscopy. Analysis of surface with electron beams.
Seminars: Mass spectrometry, modern ionisation methods.
10th week
Lectures: Microanalysis with electronic sampling. X-ray diffraction analysis. Scanning electron microskop.
Seminars: Potentiometry (numerical examples).
11th week
Lectures: Electroanalytical methods. Potentiometry. Indicator electrodes. Potentiometric setup.
Seminars: Potentiometry (numerical examples).
12th week
Lectures: Coulometry.
Seminars: Electrogravimetry (numerical examples).
13th week
Lectures: Coulometry
Seminars: Coulometry (numerical examples).
14th week
Lectures: Voltammetry.
Seminar: Voltammetry (numerical examples).
15th week
Lectures: Amperometry.
Seminars: Amperometry (numerical examples).
Experimantal part:
1. Kinetic methods of analysis, determoination of tiolic compound using kinetic manifold with spectrophotometric detector.
2. Flow injection analysis, determination of ascorbic acid by flow injection analysis and spectrophotometric detector.
3. UV/Vis spectrophotometry, spectrophotometric measurement of an equilibrium constant.
4. Atomic absorption spectroscopy, determination of metals in real samples.
5. Ions selective electrode, potentiometry, measurement of an equilibrium constant.
6. Electrogravimetric determination, determination or separation of metals.

Format of instruction:

Student responsibilities